Application of humanities curriculum by narrative-based instruction in training of graduate students in oncology / 中华医学教育探索杂志

In recent years,the application of humanities curricula has gained increasing attention gradually in Chinese medical education.For graduate students in oncology who have just been exposed to the actual clinical work,it is extremely important to develop the comprehensive abilities of physician-patient communication,humanistic care and self psychological dredging.Since 2012,we have conducted humanities curriculum by narrative-based instruction for the clinical intern graduate students in oncology at the department of surgical oncology in the First Affiliated Hospital of Xi'an Jiaotong University,and a good teaching result has been gained.This kind of humanities curricula based on open discussion could be an effective way to improve physician-patient communication ability and reduce the clinical psychology pressure for the graduate students in oncology.

Screening of cell cycle-related genes regulated by KIAA0101 in gastric cancer / 南方医科大学学报

<p><b>OBJECTIVE</b>To screen the genes related to cell cycle under regulation by KIAA0101 in gastric cancer.</p><p><b>METHODS</b>RT-PCR was used to detect the expression level of KIAA0101 gene in gastric cancer tissue and paired adjacent tissues. GO function enrichment analysis and KEGG pathway enrichment analysis were carried out using DAVID database. KEGG was used to map the pathways and the corresponding genes were analyzed. The list of genes associated with the KIAA0101 expression pattern was imported into TCGA cBioPortal to analyze the relationship between the interacting genes and generate a genetic topology map. The candidate genes were screened by RT-PCR.</p><p><b>RESULTS</b>The expression level of KIAA0101 mRNA was significantly higher in cancer tissues than in paired adjacent tissues (1.104 ± 0.379 0.421 ± 0.172; =0.0179). The system screened genes related with KIAA0101 from 478 tissues by pooled analysis of the expression intensity of all the gene probes. GO function analysis showed that the differential genes were mainly enriched in protein phosphorylation, RNA processing, cell cycle, DNA metabolism, protein transport, acetylation, apoptosis, proteolysis, and redox. The changes in the expression level of KIAA0101 mainly affect the gastric cancer-related pathways including cell cycle, spliceosome, DNA replication, and p53 signal transduction pathway. KEGG pathway maps and gene topology maps showed that the genes related to KIAA0101 (such as BUB1B, MAD2L1, CDC45, CDK1, CCNE1 and CCNB2) were also related to cell cycle. RT-PCR results confirmed significant increments of the expression levels of BUB1B, MAD2L, CDK1, CCNE1, and CCNB2 mRNA in gastric cancer tissues as compared with the paired adjacent gastric tissues ( < 0.05), but CDC45 mRNA did not show significant differential expression in gastric cancer tissues ( > 0.05).</p><p><b>CONCLUSIONS</b>KIAA0101 may affect cell cycle by regulating the expression of BUB1B, MAD2L1, CDK1, CCNE1 and CCNB2, and this finding may provide evidence for understanding how KIAA0101 affects cell cycle and for screening of tumor markers and selection of drug targets.</p>

Correlation between serum interleukin-6 to albumin ratio at admission and early outcome in patients with acute noncardioembolic ischemic stroke / 国际脑血管病杂志

Objective To investigate the correlation between serum interleukin-6 (IL-6) to albumin ratio (IAR) at admission and early outcome of patients with acute noncardioembolic ischemic stroke.Methods From January 2013 to May 2015,patients with acute noncardioembolic ischemic stroke admitted to the Department of Neurology,the First Affiliated Hospital of Xi'an Jiaotong University were enrolled retrospectively.Three months after onset,the clinical outcome were assessed by the modified Rankin scale (mRS),0-2 was good outcome,and ＞ 2 was poor outcome.The baseline clinical data,laboratory tests,and IAR in both groups were compared.Multivariate logistic regression analysis was used to determine the independent risk factors affecting early outcomes.Receiver operating characteristic (ROC) curve was used to evaluate the predictive value of IAR for early outcome.Results A total of 236 patients were enrolled in the study,including good outcome in 143 (60.6％) and poor outcome in 93 (39.4％).Univariate analysis showed that age (62.99 ± 11.34 years vs.59.62 ± 11.83 years;t =-2.176,P=0.031),serum IL-6 (37.56 ± 3.82 ng/L vs.34.82 ± 3.13 ng/L;t =-6.016,P ＜ 0.001),IAR (1.02 ± 0.08 vs.0.93 ± 0.07;t =-9.474,P＜0.001),and the proportions of patients with severe stroke (6.5％ vs.0％;x2 =8.142,P =0.012) and combined CHD (30.1％ vs.18.2％;x2 =4.542,P =0.033) of the poor outcome group were significantly higher than those of the good outcome group.Multivariate logistic regression analysis showed that high IAR at admission was an independent risk factor for early poor outcome (odds ratio,5.192,95％ confidence interval 3.283-8.213;P ＜0.001).ROC curve analysis showed the optimal cut-off value of IAR predicting for early outcome was 1.00,and the area under the ROC curve was 0.807 (95％ confidence interval 0.748 to 0.860).The sensitivity was 62.4％,the specificity was 88.1％,the positive predictive value was 69.2％,the negative predictive value was 79.3％,and the accuracy was 75.4％.Conclusions High serum IAR at admission was an independent risk factor for early poor outcome in patients with acute noncardioembolic ischemic stroke.It had a certain predictive value for the outcomes.

ATP1A3 gene mutations in patients with alternating hemiplegia of childhood / 中华儿科杂志

<p><b>OBJECTIVE</b>To analyze the ATP1A3 mutations in patients with alternating hemiplegia of childhood (AHC) and recognize its value in diagnosing atypical cases.</p><p><b>METHOD</b>Data of all AHC patients seen at Peking University First Hospital from August 2005 to November 2014 were prospectively collected. Clinical information of the AHC patients and their family members were collected and analyzed. Genomic DNAs were extracted from their peripheral blood. Mutations in ATP1A3 were screened by Sanger sequencing after PCR.</p><p><b>RESULT</b>A total of 78 AHC patients were recruited, including 50 males and 28 females. Only three patients had family history of AHC. The first family case had affected mother with AHC; the second family case was the older one of a monozygotic male twins with AHC but their parents were normal; the third family case had a sister with AHC but their parents were normal. The age of onset ranged from six hours to eight years and six months (median: 4 months). According to the Aicardi's clinical diagnostic criteria, 72 patients were considered as typical AHC cases and the other six patients were considered as atypical AHC cases for their age of onset was older than 18 months. Twenty-seven different missense ATP1A3 mutations were detected in 71 (91.0%, 71/78) patients with AHC, including 66 typical and 5 atypical cases. 11 novel ATP1A3 mutations were first reported. ATP1A3 mutations were identified in the three AHC cases with family history. Parental analysis verified that the ATP1A3 mutation of 63 patients (95.5%, 63/66) were de novo origin except lack of five unavailable maternal or paternal genomic DNA. Mutation D801N was found in 20 cases (28.2%), and E815K in 12 cases (16.9%). In the six atypical AHC patients, ATP1A3 mutations were detected in five of them.</p><p><b>CONCLUSION</b>ATP1A3 was the major causative gene of AHC, and mutations were identified as de novo mostly. ATP1A3 mutations in AHC had mutational hotspot, and the most common mutations were D801N and E815K. ATP1A3 mutation screening is helpful for the genetic and definite diagnosis of the atypical AHC cases.</p>

Small-cell neuroendocrine carcinoma of the stomach: a clinical and prognostic analysis of 21 cases / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate and improve the diagnosis and management of small-cell neuroendocrine carcinoma of the stomach (SCNECS).</p><p><b>METHODS</b>The clinicopathological information and survival data of 21 cases of SCNECS treated in our hospital from January 2003 to December 2012 were analyzed retrospectively.</p><p><b>RESULTS</b>The median survival time of the 21 cases was (12.1±1.6) months. The 1-year overall survival rate of the patients was 33.3%. Univariate analysis showed that the risk factors of survival were tumor size, lymph node status, tumor stage, treatment and radical operation or not (P<0.05 for all). Multivariate analysis indicated that independent risk factors were tumor size ≥4.6 cm, lymph node metastasis and tumor stage III/IV (P<0.05 for all). Radical operation and comprehensive treatment (surgery + postoperative chemotherapy) were independent protective factors (all P<0.05).</p><p><b>CONCLUSIONS</b>SCNECS is a rare malignant tumor with early metastasis and poor prognosis. Tumor size, stage, lymph node status, and treatment have potential impact on the prognosis. Comprehensive treatment based on radical operation may improve the survival of SCNECS patients.</p>

Method for Determination of Bisphenol A in Drinking Water by Liquid Chromatography-Tandem Mass Spectrometry / 环境与健康杂志

Objective To develop a rapid and sensitive method for the determination of bisphenol A in drinking water by liquid chromatography-electrospray tandem mass spectrometry. Methods Bisphenol A was extracted from the drinking water sample by a SEP-PAK C18 column,eluted with methanol and concentrated with a rotary evaporator. The extract was analyzed by liquid chromatography-tandem mass spectrometry with electrospray ionization. Results The calibration curve of bisphenol A was linear in the range of 5 -100 ng/ml,the linear equation was y =6 796.61x -8 655.64 and the correlation coefficients of linear calibration curve was 0.999 2. The limit of detection (S/N=3) and the limit of quantification (S/N=10) of bisphenol A were 0.007 5 ng/ml and 0.025 ng/ml,respectively. The rates of recovery of bisphenol A were from 83.46% to 94.00% and the relative standard deviation was 3.06% -4.60%. Conclusion The method is simple,rapid,accurate and is applicable to the qualitative and quantitative determination of bisphenol A in drinking water.